Cell Cycle and Cancer Laboratory, BHU

About Us

Research Focus

The research focus of our lab revolves around understanding an essential regulatory process of a cells' life, cell cycle. Cell cycle is divided into two phases-the interphase and the mitotic phase. Cell spends most of its life in the interphase which is further categorized into G1, S, and G2 phase. G1 and G2 are the gap phases where a cell gets prepared for DNA replication and division respectively. These two gap phases are connected by a S phase where DNA containing genetic information gets replicated. Irreparable errors in this phase causes accumulation of mutations leading to cancer.

We are looking into the regulation of cell cycle in cancerous cells and want to see how oncogenic proteins affect the progression of cell cycle and what is the fate of replication in the same. We are also very much interested in understanding signaling pathways involved in transformation of normal cells into cancerous cells.

Interests

Our team is involved in understanding the basic mechanisms of S phase of cell cycle. In particular, we are more interested in some specific transcription factors and micro RNAs which may or may not affect the major regulators of the cell cycle. Also, we expertise in understanding the structural and functional aspects of major cell cycle regulators and their role in therapeutics. We use molecular biology, cryo-EM and various biochemical and biophysical approaches to find out the molecular mechanism underpinning the regulation of cell cycle.

Team Members

Garima Singh (Ph.D. Awarded)

Completed her PhD on "Role of miRNAs in Cell Cycle Regulation in Cervical Cancer Cells."

Email: garima.singh10@bhu.ac.in

Gajender Singh (Ph.D. Scholar)

Cryo-EM Study of Essential Mammalian Protein: Transcription Termination Factor 1 (TTF1) and its Role in Cancer Etiology

Email: gajender97@bhu.ac.in

Sonika Kumari Sharma (Ph.D. Scholar)

Role of microRNAs in Regulating Cell Cycle in Gallbladder Cancer

Email: sonika3094@bhu.ac.in

Neelu Mishra (CSIR-JRF)

Role of mi-RNA in DNA Damage Repair and Cell Cycle Progression

Email: neelu1403@bhu.ac.in

Recent Publications

Singh, G., Sharma, S. K., Dorata, A., & Singh, S. K. (2023). miR-17~ 92 suppresses proliferation and invasion of cervical cancer cells by inhibiting cell cycle regulator Cdt2. Discover Oncology, 14(1), 172.
Tiwari, K., Bose, S., Mishra, N., & Singh, S. K. (2023). Novel Function of Transcription Factor TTF1 in UV Mediated DNA Damage Repair in Mammalian Cells. bioRxiv, 2023-05.
Tiwari, K., Singh, G., & Singh, S. K. (2022). Purification and Structural Characterization of N-Terminal 190 Amino Acid Deleted Essential Mammalian Protein; Transcription Termination Factor 1. ACS omega, 7(49), 45165-45173.
Kumud Tiwari, Aditi Gangopadhyay, Gajender Singh, Vinay Kumar Singh & Samarendra Kumar Singh (2022) Ab initio modelling of an essential mammalian protein: Transcription Termination Factor 1 (TTF1), Journal of Biomolecular Structure and Dynamics, DOI: 10.1080/07391102.2022.2109754
Tiwari K, Singh G, Singh G, Sharma SK, Singh SK. Industrial biotechnology: An Indian perspective. J App Biol Biotech. 2022;10(5):22-33. DOI: 10.7324/JABB.2022.100503
Singh G, Sharma SK, Singh SK. miR-34a negatively regulates cell cycle factor Cdt2/DTL in HPV infected cervical cancer cells. BMC Cancer. 2022 Jul 15;22(1):777. doi: 10.1186/s12885-022-09879-5. PMID: 35840896; PMCID: PMC9288023.
Shrivastava, A., Singh, G., Tiwari, K., Mishra, S. P., Pradhan, S., Agarwal, L. M., & Singh, S. K. (2020). A study for evaluating clinical relevance of circulating cell-free DNA in cervical cancer. J Cancer Res Ther. DOI: 10.4103/jcrt.JCRT_415_20
Garima S, Kumud T, Singh SK (2020) Curing of Mammalian Cell Lines from Severe Bacterial Contamination. J Appl Microb Res. Vol: 3 Issu: 2 (01-06).
Kiran, S., Dar, A., Singh, S. K., Lee, K. Y., & Dutta, A. (2018). The deubiquitinase USP46 is essential for proliferation and tumor growth of HPV-transformed cancers. Molecular cell, 72(5), 823-835. https://doi.org/10.1016/j.molcel.2018.09.019.
Etsuko Shibata, Manjari Kiran, Yoshiyuki Shibata, Samarendra Singh, Shashi Kiran, Anindya Dutta (2016) Two subunits of human ORC are dispensable for DNA replication and proliferation eLife 5:e19084. https://doi.org/10.7554/eLife.19084
Jaiswal, R., Choudhury, M., Zaman, S., Singh, S., Santosh, V., Bastia, D., & Escalante, C. R. (2016). Functional architecture of the Reb1-Ter complex of Schizosaccharomyces pombe. Proceedings of the National Academy of Sciences, 113(16), E2267-E2276. https://doi.org/10.1073/pnas.1525465113
Singh, S. K., & Gellert, M. (2015). Role of RAG1 autoubiquitination in V (D) J recombination. Proceedings of the National Academy of Sciences, 112(28), 8579-8583. https://doi.org/10.1073/pnas.1510464112
Um, J. H., Brown, A. L., Singh, S. K., Chen, Y., Gucek, M., Lee, B. S., ... & Chung, J. H. (2013). Metabolic sensor AMPK directly phosphorylates RAG1 protein and regulates V (D) J recombination. Proceedings of the National Academy of Sciences, 110(24), 9873-9878. https://doi.org/10.1073/pnas.1307928110
Bastia, D, Singh, S.K., (2011) Chromosome kissing and modulation of replication termination. Bioarchitecture. 1, 24-28.
Singh, S.K., Sabatinos, S., Forsburg, S., Bastia, D. (2010) Regulation of replication termination in Schizosaccharomyces pombe by terminator protein mediated action at a distance. Cell. 142, 868-78.
Singh, S.K.*, Singh, A., Banerjee, P.C. (2010) Plasmid encoded AcrAB-TolC tripartite multidrug-efflux system in Acidiphilium symbioticum H8. Curr. Microbiol. 61(3), 163-168. *Corresponding Author
Saxena, M., Singh, S.K., Zzaman, S., Bastia, D. (2010) Investigations of π Initiator Protein-mediated Interaction between Replication Origins α and γ of the Plasmid R6K. J. Biol. Chem. 285, 5695-5704.
Singh, S.K., Banerjee, P.C. (2007) Nucleotide sequence analysis of cryptic plasmid pAM5 from Acidiphilium multivorum. Plasmid 58, 101-114.
Saha, A., Singh, S.K., Ghosh, A.K., Banerjee, P.C. (2007) Detection of Insertion sequences in chromosome of an Acidocella strain. Res. J. Microbiol. 2 (10), 756-762.
Singh, S.K., Singh, A., Ghosh, A.K., Banerjee, P.C. (2007) Plasmid encoded membrane protein TolC responsible for tolerance to toxic hydrophobic agents and organic solvent in Acidiphilium symbioticum H8. In: Mineral Biotechnology (Mishra BK, Shukla LB, and Rao KS, Eds) pp. 288-295.
Singh, S.K., Banerjee, P.C. (2006) High-yielding plasmid extraction method from acidophilic heterotrophic bacteria of the genus Acidiphilium. Anal. Biochem. 356, 229-234.

"A place where thoughts and science never die."

About Us

Research Focus

Interests

Principal Investigator

Dr. Samarendra K Singh

Team Members

Garima Singh (Ph.D. Awarded)

Gajender Singh (Ph.D. Scholar)

Sonika Kumari Sharma (Ph.D. Scholar)

Neelu Mishra (CSIR-JRF)

Alumni

Dr. Kumud Tiwari

M.Sc. Dissertees

Akshay Vijay Rathod

Akshay Vijay Rathod

Akshay Vijay Rathod

Manshi Kumari

Akshay Vijay Rathod

Akshay Vijay Rathod

Prashant Prakash

Akshay Vijay Rathod

Prashant Prakash

Simran Mathur

Akshay Vijay Rathod

Prashant Prakash

Aastha Dorata

Aastha Dorata

Aastha Dorata

Saloni Khatri

Aastha Dorata

Aastha Dorata

Snehendu Bose

Aastha Dorata

Snehendu Bose

Trainees/interns

Anamika Singh

Smrutimayee Prusty

Sudiksha Surabhi

Sauranil Guha

Priyanka

Supriya

Simran Negi

Recent Publications

One Opening for Ph.D. Research Scholar Position

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